Đồ án tốt nghiệp - Phytochemical, antioxidant capacity and antifungal property screening of PESEE

i

Phytochemical, antioxidant capacity and antifungal property
screening of Passiflora edulis Seed Ethanolic Extract

MISS NGUYEN HUYNH TIEN
STUDENT NO. 15116051

A THESIS SUBMITTED IN PARTIAL FULFILMENT OF THE REQUIREMENT FOR
THE DEGREE OF BACHELOR OF SCIENCE (FOOD TECHNOLOGY)
SCHOOL OF BIORESOURCES AND TCHNOLOGY
KING MONGKUT‘S UNIVERSITY OF TECHNOLOGY THONBURI
HO CHI MINH CITY UNIVERSITY OF TECHNOLOGY AND EDUCATION
2019

ii

Phytochemical, antioxidant capacity and antifungal property
screening of Passiflora edulis Seed Ethanolic Extract

Miss Nguyen Huynh Tien
(Intership student)

A Thesis Submitted in Partial Fulfilment of the Requirement for
the Degree of Bachelor of Science (Food Technology)
Ho Chi Minh City University of Technology and Education
2019

Thesis Committee
…………………………………………………

Chairman of Thesis Commitee

(Asst. Prof. Songsin Photchanachai, Dr. Agr. Sci.)
…………………………………………………

Member and Mentor

(Ms. Wissanee Pola, Ph.D. student)

Coppyright Reserved

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Thesis Title

Phytochemical, antioxidant capacity and antifungal property
screening of Passiflora edulis Seed Ethanolic Extract

Candidate

Miss Nguyen Huynh Tien

Thesis Advisor

Assoc. Prof. Songsin Photchanachai

Field of Study

Chemistry and Food Technology/ Postharvest Technology

Faculty

Faculty for high quality training/ School of Bioresources and
Technology

Academic year

2019

Abstract
Passion fruit seeds are byproduct from juice production and abundant in nutritional and
health benefits. The objective of this study was to evaluate total phenolic content
(TPC), antioxidant capacity and antifungal activities of ethanolic extract of passion
fruit seeds. The seeds were grounded and extracted with 50% and 70% ethanol. The
TPC was evaluated by Folin-Ciocalteau assay. Antioxidant capacities were measured
by free radical scavenging activity (DPPH 2,2-diphenyl-1-picrylhydrazyl), ferric
reducing antioxidant power (FRAP) and antifungal acvity against Aspergillus niger
using the radial growth technique. Results showed that the TPC and antioxidant
capacities of the crude extraction using 70% ethanol are significantly higher than that
of the 50% ethanol. Moreover, the ethanolic extract of P.edulis seed cake showed
antifungal activities against important fungal foodborne pathogen ( Aspergillus niger).
Conversely, the concentration of the solvent did not influence the crude yield. These
findings demonstrated that the 70% ethanol would be an appropriate concentratio
...

...
J., ... &amp Li, H. B. 2017.
Natural antioxidants in foods and medicinal plants: Extraction, assessment and
resources. International journal of molecular sciences, 18(1), 96.
Y. Matsumoto, N. Gotoh, S. Sano, K. Sugiyama, T. Ito, Y. Abe, Y. Katano, A.
Ishihata, ―Effects of Scirpusin B, A polyphenol in passion fruit seeds, on the
coronary circulation of the isolated perfused rat heart‖, International Journal of
Medical Research &amp Health Sciences, vol. 3, pp. 547-553, 2014
Youle, R. J., &amp Huang, A. H. 1981. Occurrence of low molecular weight and high
cysteine

containing

albumin

storage

proteins

in

oilseeds

of

diverse

species. American Journal of Botany, 68(1),pp 44-48.
Yu, L., Haley, S., Perret, J., Harris, M., Wilson, J., &amp Qian, M. 2002. Free radical
scavenging properties of wheat extracts. Journal of Agricultural and Food
Chemistry, 50(6), pp 1619-1624
Zeraik, M. L., Pereira, C. A., Zuin, V. G., &amp Yariwake, J. H. 2010. Maracujá: um
alimento funcional. Revista Brasileira de farmacognosia, 20(3), pp 459-471.

48

APPENDIX A
Table 4. Total phenolic content of crude extracts using 50% and 70% ethanol.
Treatments
Ethanol 50%
Ethanol 70%
p

The total phenolic content(mmol GAE/100 g FW)
252.60 ± 6.741)
287.29 ± 26.04
*2)

1)

Values are means ± SD (n=4)

2)

Significant differences between samples are presented as * for p≤0.05

Table 5. Antioxidant activity of crude is extracted using ethanol 50% and
ethanol 70% determined by DPPH, FRAP assays.
Treatments

DPPH (%)

Ethanol 50%
Ethanol 70%
p

36.74 ± 1.481)
48.97 ± 3.151)
**2)

FRAP (mmol
Trolox/100 g FW)
56.95 ± 8.9921)
116.99 ± 9.161)
**2)

1)

Values are means ± SD (n=4)

2)

Significant differences between samples are presented as ** for p≤0.05

Table 6. Percentage inhibition of mycelial growth of crude extract from Passion
fruit seed using 70% and 50% ethanol.
TRT

Day 1

Day 2

Day 3

Day 4

Day 5

70% Ethanol 0.5%

37.1429

3.93939

-4.7228

-5.6669

-6.5565

50% Ethanol 0.5%

34.2857

5.75758

-1.232

-5.6669

-7.7367

Possivetive

13.6857

6.06061

2.95688

5.47902

7.39575

49

Table 7. The growthing of fungi in four different mediums.
Treatments

1)

Diameter (cm)
Day 1

Day 2

Day 3

Day 4

Day 5

Ethanol
50%
Ethanol
70%
DMSO

1.38c1)

3.11a1)

4.96a1)

6.75a1)

8.24a1)

1.32c1)

3.17a1)

5.1a1)

6.75a1)

8.12a1)

1.65b

3.24a

4.67b

6.34ab

7.60b

Positive

1.81a

3.10a

4.72b

6.04b

7.06c

Values are means ± SD (n=5)

The same letter are not significantly different.